7O4Y
m971 Fab in complex with anti-Kappa VHH domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979510 |
Spacegroup name | P 31 |
Unit cell lengths | 73.998, 73.998, 98.808 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.127 - 1.600 |
R-factor | 0.2198 |
Rwork | 0.219 |
R-free | 0.24590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ani |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.127 | 39.127 | 1.700 |
High resolution limit [Å] | 1.600 | 4.760 | 1.600 |
Rmerge | 0.045 | 0.027 | 0.537 |
Rmeas | 0.051 | 0.031 | 0.620 |
Total number of observations | 329897 | ||
Number of reflections | 79907 | 3004 | 12852 |
<I/σ(I)> | 17.31 | 50.92 | 2.36 |
Completeness [%] | 99.8 | 99.7 | 99.4 |
Redundancy | 4.129 | 4.23 | 3.969 |
CC(1/2) | 0.999 | 0.998 | 0.777 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M lithium acetate, 20% PEG 3350 |