7O3U
The crystal structure of obelin from Obelia longissima bound with v-coelenterazine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 42.880, 42.880, 375.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.140 - 1.800 |
| R-factor | 0.2413 |
| Rwork | 0.240 |
| R-free | 0.26410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qv0 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18rc1_3777) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.099 | 0.705 |
| Rmeas | 0.106 | 0.771 |
| Number of reflections | 35638 | 5610 |
| <I/σ(I)> | 12.77 | 1.86 |
| Completeness [%] | 99.5 | 97.2 |
| Redundancy | 8.3 | 5.7 |
| CC(1/2) | 0.998 | 0.896 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 2.2 M DL-Malic acid |
