7O3I
Cystal structure of Zymogen Granule Protein 16 (ZG16)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9724 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.927, 55.996, 58.115 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.320 - 1.500 |
| R-factor | 0.1732 |
| Rwork | 0.171 |
| R-free | 0.20750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3apa |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.972 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.323 | 1.554 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 20346 | 2016 |
| <I/σ(I)> | 10.87 | |
| Completeness [%] | 97.0 | 97.25 |
| Redundancy | 3.8 | 3.7 |
| CC(1/2) | 0.986 | 0.621 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.0 M ammonium sulfate, 0.1 M BIS-Tris, pH 5.5, 1 % w/v PEG 3350 |
