7O1F

PCNA from Chaetomium thermophilum in complex with PolD4 PIP peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2020-12-02
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54178
Spacegroup name	P 1
Unit cell lengths	71.891, 84.711, 84.890
Unit cell angles	60.94, 89.60, 81.22

Refinement procedure

Resolution	30.390 - 2.450
R-factor	0.2221
Rwork	0.220
R-free	0.26190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7o1e
RMSD bond length	0.007
RMSD bond angle	1.461
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.390	30.390	2.520
High resolution limit [Å]	2.450	10.680	2.450
Rmerge	0.130	0.048	1.368
Rmeas	0.151	0.055	1.610
Rpim	0.076	0.028	0.841
Total number of observations	230753	2790	16225
Number of reflections	59727	711	4557
<I/σ(I)>	7.2	23.9	0.8
Completeness [%]	94.2	92.7	92.1
Redundancy	3.9	3.9	3.6
CC(1/2)	0.994	0.996	0.406

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2 M Calcium acetate hydrate, 0.1 M Sodium cacodylate, pH 6.5, 36 % v/v PEG 300