7O0A

Bdellovibrio bacteriovorus PGI in P1211 spacegroup

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	137.920, 118.100, 138.980
Unit cell angles	90.00, 118.41, 90.00

Refinement procedure

Resolution	59.540 - 1.740
R-factor	0.2238
Rwork	0.222
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7nss
RMSD bond length	0.007
RMSD bond angle	1.341
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.540	1.770
High resolution limit [Å]	1.740	1.740
Rmerge	0.090	0.607
Rmeas	0.116	0.788
Rpim	0.073	0.495
Number of reflections	395364	29299
<I/σ(I)>	8.7	1.5
Completeness [%]	98.8
Redundancy	3.9
CC(1/2)	0.982	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	291	0.2 M ammonium acetate 0.1 M sodium acetate pH4.0 15% w/v PEG 4000