7O06

Crystal structure of the N-terminal domain of CEP164(1-109) bound to camelid nanobody 10Z

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97942
Spacegroup name	P 1 21 1
Unit cell lengths	50.852, 70.734, 61.969
Unit cell angles	90.00, 90.09, 90.00

Refinement procedure

Resolution	61.970 - 1.600
R-factor	0.1819
Rwork	0.180
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vlv
RMSD bond length	0.006
RMSD bond angle	0.797
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.7.0009)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.970	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.097	1.463
Rpim	0.040	0.627
Number of reflections	57658	2820
<I/σ(I)>	9.9	1.7
Completeness [%]	99.4	98.9
Redundancy	6.7	6.3
CC(1/2)	0.998	0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	0.1 M Hepes, pH 7.5, 1.26 M ammonium sulfate