7O06
Crystal structure of the N-terminal domain of CEP164(1-109) bound to camelid nanobody 10Z
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.852, 70.734, 61.969 |
| Unit cell angles | 90.00, 90.09, 90.00 |
Refinement procedure
| Resolution | 61.970 - 1.600 |
| R-factor | 0.1819 |
| Rwork | 0.180 |
| R-free | 0.21080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vlv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.797 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0009) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.970 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.097 | 1.463 |
| Rpim | 0.040 | 0.627 |
| Number of reflections | 57658 | 2820 |
| <I/σ(I)> | 9.9 | 1.7 |
| Completeness [%] | 99.4 | 98.9 |
| Redundancy | 6.7 | 6.3 |
| CC(1/2) | 0.998 | 0.649 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 292 | 0.1 M Hepes, pH 7.5, 1.26 M ammonium sulfate |
