7NZZ

Ras1 guanine nucleotide exchange factor Cdc25 (REM and catalytic domains)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	48.647, 107.214, 196.896
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.220 - 2.450
R-factor	0.1967
Rwork	0.194
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4us0
RMSD bond length	0.002
RMSD bond angle	0.439
Data reduction software	XDS (VERSION MAR 15, 2019 BUILT=20190806)
Data scaling software	Aimless (Version 0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.220	2.496
High resolution limit [Å]	2.450	2.454
Rmerge	0.147	1.504
Rmeas	0.165	1.685
Rpim	0.073	0.750
Number of reflections	38604	1893
<I/σ(I)>	8.5	1.2
Completeness [%]	99.9	100
Redundancy	4.9	5
CC(1/2)	0.995	0.454

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	23% (w/v) PEG 3350, 0.25 M sodium malonate dibasic monohydrate