7NZP
D-lyxose isomerase from the hyperthermophilic archaeon Thermofilum sp complexed with D-fructose
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-07 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.958, 86.647, 53.837 |
| Unit cell angles | 90.00, 112.27, 90.00 |
Refinement procedure
| Resolution | 49.819 - 1.345 |
| Rwork | 0.184 |
| R-free | 0.21260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y0o |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.838 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.650 | 1.370 |
| High resolution limit [Å] | 1.345 | 1.345 |
| Number of reflections | 88231 | 4299 |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.4 | |
| CC(1/2) | 0.999 | 0.333 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.5 | 291 | 200 mM ammonium acetate, 100 mM Bis-Tris pH 5.5, 25 % w/v PEG 3350, 15 mM fructose |
