7NZA
Structure of OBP1 from Varroa destructor, form P2<1>
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.604, 112.959, 41.817 |
| Unit cell angles | 90.00, 98.95, 90.00 |
Refinement procedure
| Resolution | 38.830 - 1.199 |
| R-factor | 0.18056 |
| Rwork | 0.179 |
| R-free | 0.20145 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7nyj |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.197 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.100 | 1.270 |
| High resolution limit [Å] | 1.199 | 1.190 |
| Rmerge | 0.065 | 0.885 |
| Number of reflections | 87891 | 13358 |
| <I/σ(I)> | 13.15 | 1.19 |
| Completeness [%] | 97.2 | 91.4 |
| Redundancy | 7 | 6.4 |
| CC(1/2) | 0.998 | 0.788 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 2/3 of the initial crystallization conditions (CHES pH9.5, 0.1M, 30% PEG3000 w/v ) and 1/3 of ammonium sulfate screen (0.2 M sodium nitrate, 2.2M ammonium sulfate). |
