7NYQ

Crystal structure of the Mei-P26 NHL domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9184
Spacegroup name	P 1 21 1
Unit cell lengths	34.710, 116.500, 64.700
Unit cell angles	90.00, 96.36, 90.00

Refinement procedure

Resolution	43.170 - 1.600
R-factor	0.1964
Rwork	0.196
R-free	0.21460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q7f
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.640
High resolution limit [Å]	1.599	1.600
Number of reflections	66500	4807
<I/σ(I)>	10.15	0.85
Completeness [%]	98.8
Redundancy	3.82
CC(1/2)	0.999	0.459

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	KSCN, PEG3350