Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96546 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 71.399, 82.128, 46.849 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.883 - 1.450 |
| Rwork | 0.157 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fpe |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.689 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (7.1.007) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.883 | 1.480 |
| High resolution limit [Å] | 1.350 | 1.356 |
| Number of reflections | 41308 | 2066 |
| <I/σ(I)> | 9.5 | 1.5 |
| Completeness [%] | 91.8 | |
| Redundancy | 4.6 | |
| CC(1/2) | 0.993 | 0.610 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293.15 | 0.1 M HEPES pH 7.5; 1-5 PEG 400; 2-2.5 M Ammonium sulphate |
