7NY6

Crystal structure of the Capsaspora owczarzaki macroH2A macrodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	41.030, 44.071, 44.860
Unit cell angles	90.00, 92.36, 90.00

Refinement procedure

Resolution	41.000 - 1.340
R-factor	0.1841
Rwork	0.183
R-free	0.21190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iid
RMSD bond length	0.010
RMSD bond angle	1.285
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.000	1.386
High resolution limit [Å]	1.338	1.338
Rmerge	0.067	0.610
Rmeas	0.074	0.740
Number of reflections	32600	1630
<I/σ(I)>	13.69	1.11
Completeness [%]	90.0	45.63
Redundancy	5.9	2.5
CC(1/2)	0.990	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1M Bis-Tris (pH 5.5) 25% (w/v) PEG3350