7NY6
Crystal structure of the Capsaspora owczarzaki macroH2A macrodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.030, 44.071, 44.860 |
| Unit cell angles | 90.00, 92.36, 90.00 |
Refinement procedure
| Resolution | 41.000 - 1.340 |
| R-factor | 0.1841 |
| Rwork | 0.183 |
| R-free | 0.21190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iid |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.285 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 1.386 |
| High resolution limit [Å] | 1.338 | 1.338 |
| Rmerge | 0.067 | 0.610 |
| Rmeas | 0.074 | 0.740 |
| Number of reflections | 32600 | 1630 |
| <I/σ(I)> | 13.69 | 1.11 |
| Completeness [%] | 90.0 | 45.63 |
| Redundancy | 5.9 | 2.5 |
| CC(1/2) | 0.990 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1M Bis-Tris (pH 5.5) 25% (w/v) PEG3350 |
