7NXP

Structure of the C-terminal domain of the pUL77 capsid protein from human cytomegalovirus (HCMV)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978570
Spacegroup name	P 41 2 2
Unit cell lengths	163.907, 163.907, 50.965
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.650 - 1.896
R-factor	0.1923
Rwork	0.192
R-free	0.20810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f5u
RMSD bond length	0.010
RMSD bond angle	0.880
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.010
High resolution limit [Å]	1.896	1.896
Number of reflections	55350	8600
<I/σ(I)>	16.66
Completeness [%]	99.5
Redundancy	8.6
CC(1/2)	0.999	0.659

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.08 M Na-Cacodylate pH 6.5 16% PEG 8000 20 % glycerol