7NXP
Structure of the C-terminal domain of the pUL77 capsid protein from human cytomegalovirus (HCMV)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978570 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 163.907, 163.907, 50.965 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.650 - 1.896 |
| R-factor | 0.1923 |
| Rwork | 0.192 |
| R-free | 0.20810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f5u |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.880 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.896 | 1.896 |
| Number of reflections | 55350 | 8600 |
| <I/σ(I)> | 16.66 | |
| Completeness [%] | 99.5 | |
| Redundancy | 8.6 | |
| CC(1/2) | 0.999 | 0.659 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.08 M Na-Cacodylate pH 6.5 16% PEG 8000 20 % glycerol |
