7NX2

Unbound antigen-binding fragment (FAb) 324

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	81.240, 87.090, 126.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.550 - 1.470
R-factor	0.1684
Rwork	0.168
R-free	0.20570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nuz
RMSD bond length	0.008
RMSD bond angle	1.025
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.760	1.560
High resolution limit [Å]	1.470	1.470
Rmeas	0.040	0.390
Number of reflections	147145	19382
<I/σ(I)>	17.32
Completeness [%]	99.2	79
Redundancy	3.82
CC(1/2)	0.990	0.420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	1.5M (NH4)2SO4 40 mM Glycine pH 9.5