7NVK
Crystal structure of UBA5 fragment fused to the N-terminus of UFC1
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2019-12-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 64 |
| Unit cell lengths | 84.630, 84.630, 60.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.780 - 2.651 |
| Rwork | 0.216 |
| R-free | 0.26380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z6o |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.454 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.780 | 2.780 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmeas | 0.112 | 0.600 |
| Number of reflections | 7314 | 953 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.4 | 21.4 |
| CC(1/2) | 0.990 | 0.292 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2% (v/v) Tacsimate pH 7.0, 20% PEG 3350, 0.1M HEPES pH 7.5, 6mM zinc sulfate |
