7NVJ

Crystal structure of UFC1 Y110A & F121A

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	PIXEL
Collection date	2021-02-23
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 41 21 2
Unit cell lengths	46.161, 46.161, 143.755
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.240 - 2.200
Rwork	0.193
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z6p
RMSD bond length	0.009
RMSD bond angle	1.468
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.240	2.279
High resolution limit [Å]	2.200	2.200
Rmeas	0.153	0.710
Number of reflections	8491	804
<I/σ(I)>	13.33	2.7
Completeness [%]	99.8	99.14
Redundancy	1.2	1.1
CC(1/2)	0.990	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1M HEPES pH 7.5, 2M Ammonium sulfate