7NUV

Crystal structure of the Aux2pLS20 tetramerization domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97916
Spacegroup name	P 4 21 2
Unit cell lengths	44.840, 44.840, 141.153
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.050 - 1.760
R-factor	0.2078
Rwork	0.206
R-free	0.24170
Structure solution method	AB INITIO PHASING
RMSD bond length	0.007
RMSD bond angle	0.912
Phasing software	Arcimboldo
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.050	44.417	1.670
High resolution limit [Å]	1.640	4.455	1.640
Rmerge	0.034		1.144
Rmeas	0.041
Number of reflections	28809	1769	1717
<I/σ(I)>	14.7	37.6	0.9
Completeness [%]	82.6		96.4
Redundancy	3.4
CC(1/2)	0.999	0.998	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	Protein concentrated to a final concentration of 10 mg/ml in 20mM Tris (pH8.0), 300mM NaCl. Crystals obtained by the sitting-drop vapor-diffusion method at 291K, by equilibration of drops of 0.1uL protein + 0.1uL crystallization buffer ( 0.2 M Magnesium chloride hexahydrate, 20% w/v Polyethylene glycol 3,350) against 50uL of the crystallization buffer