7NS7
Human L-alanine:glyoxylate aminotransferase minor allele variant: AGXT-Mi (P11L-I340M)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 41 |
| Unit cell lengths | 90.590, 90.590, 140.858 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.060 - 2.200 |
| R-factor | 0.2455 |
| Rwork | 0.241 |
| R-free | 0.28940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f9s |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.589 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 64.060 | 64.060 | 2.260 |
| High resolution limit [Å] | 2.200 | 9.590 | 2.200 |
| Rmerge | 0.109 | 0.054 | 0.369 |
| Rmeas | 0.125 | 0.062 | 0.429 |
| Rpim | 0.061 | 0.029 | 0.213 |
| Total number of observations | 214921 | 2813 | 16842 |
| Number of reflections | 57290 | 716 | 4437 |
| <I/σ(I)> | 9.3 | 20.6 | 3.6 |
| Completeness [%] | 99.6 | 98.4 | 99.5 |
| Redundancy | 3.8 | 3.9 | 3.8 |
| CC(1/2) | 0.992 | 0.995 | 0.788 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 1:1 mixing of: Protein: 0.04M AGT-Mi solution in 0.1M KPhO Precipitant: 0.03M MgCl2 , 0.03M CaCl2 , 0.05M HEPES 0.05M MOPS at a final pH 7.5, 40% v/v Ethylene glycol; 20% w/v PEG 8K |
