7NS5

Structure of yeast Fbp1 (Fructose-1,6-bisphosphatase 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-08-26
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	58.018, 133.794, 171.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.220 - 1.950
R-factor	0.2103
Rwork	0.208
R-free	0.24240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fta
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.224	2.016
High resolution limit [Å]	1.946	1.950
Rmerge	0.096
Rpim	0.040
Number of reflections	98528	15496
<I/σ(I)>	19.73
Completeness [%]	99.7
Redundancy	12.6
CC(1/2)		0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	16% PEG 3350, 0.2 M MgCl2 and 0.1 M Bis-Tris pH 6