7NRU
Structure of a natural chimera of meningococcal factor H binding protein belonging to NL096 strain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-30 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.99987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.680, 42.610, 44.410 |
| Unit cell angles | 90.00, 100.20, 90.00 |
Refinement procedure
| Resolution | 23.368 - 1.220 |
| R-factor | 0.143044838589 |
| Rwork | 0.142 |
| R-free | 0.16591 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kvd |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.157 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.370 | 1.264 |
| High resolution limit [Å] | 1.219 | 1.220 |
| Rmerge | 0.063 | 0.200 |
| Number of reflections | 65693 | 9280 |
| <I/σ(I)> | 10.5 | 4.4 |
| Completeness [%] | 95.2 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 294.15 | 0.1M tri-sodium citrate, 3.2M ammonium sulfate |
