7NPE

Vibrio cholerae ParA2-ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-07-21
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	P 61 2 2
Unit cell lengths	199.205, 199.205, 260.066
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.590 - 3.200
R-factor	0.24
Rwork	0.238
R-free	0.27790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7npd
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.590	3.298
High resolution limit [Å]	3.180	3.184
Rmerge	0.021	0.564
Number of reflections	49709	3364
<I/σ(I)>	0.98
Completeness [%]	96.4	66.68
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	24 %w/v PEG 1500, 20% w/v glycerol