7NPD
Vibiro cholerae ParA2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-07-21 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 63.247, 63.247, 214.373 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.310 - 2.600 |
| R-factor | 0.2803 |
| Rwork | 0.274 |
| R-free | 0.33670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ez7 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.310 | 2.694 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.017 | 0.375 |
| Number of reflections | 14628 | 689 |
| <I/σ(I)> | 36.81 | |
| Completeness [%] | 91.0 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M tri-Sodium citrate pH 5.5 20% w/v PEG 3000 |
