Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 51.706, 27.691, 64.521 |
| Unit cell angles | 90.00, 101.24, 90.00 |
Refinement procedure
| Resolution | 43.980 - 1.250 |
| Rwork | 0.178 |
| R-free | 0.22720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yxf |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.263 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.980 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rpim | 0.032 | 0.927 |
| Number of reflections | 24565 | 988 |
| <I/σ(I)> | 14.6 | 0.9 |
| Completeness [%] | 98.0 | 81.4 |
| Redundancy | 7 | 5.3 |
| CC(1/2) | 0.999 | 0.448 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 100 nL mother liquor / 200 nL protein 15% Glycerol, 0.1 M NaAcetate pH 4.5-5.5, 28-32%PEG 4000, 0.2 M NH4Acetate |
