7NM8
The crystal structure of the antimycin pathway standalone ketoreductase, AntM, in complex with NADPH
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-14 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 92.223, 92.223, 113.751 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.360 - 1.700 |
| Rwork | 0.199 |
| R-free | 0.22420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4osp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.657 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.360 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 33683 | 1652 |
| <I/σ(I)> | 24.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 21.2 | |
| CC(1/2) | 1.000 | 0.958 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298.15 | 0.1 M Tris, pH 8.5, 5% w/v PEG 8K, 20% v/v PEG 300, 10% v/v glycerol |
