7NLJ
Complex of rice blast (Magnaporthe oryzae) effector protein APikL2A with host target sHMA25 from Setaria italica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.208, 46.684, 88.953 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.040 - 1.800 |
| R-factor | 0.2012 |
| Rwork | 0.199 |
| R-free | 0.24450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a6w |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.592 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.039 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.058 | |
| Number of reflections | 18505 | 1060 |
| <I/σ(I)> | 21.5 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13 | 12.7 |
| CC(1/2) | 0.990 | 0.798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | Morpheus well H10 Molecular Dimensions. 0.1 M Amino acids, 0.1 M Buffer System 3, 50% v/v Precipitant Mix 2 |
