7NLG

S. cerevisiae Ty1 p22 restriction factor, Gag CA-CTD, AUG2 variant A273V mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-22
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97951
Spacegroup name	P 65 2 2
Unit cell lengths	279.873, 279.873, 39.923
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	91.610 - 3.528
R-factor	0.2268
Rwork	0.225
R-free	0.27230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Ty1 p22
Data reduction software	DIALS (1.14.13)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.610	3.590
High resolution limit [Å]	3.528	3.530
Rmeas	0.146	2.379
Rpim	0.030	0.430
Number of reflections	11994	596
<I/σ(I)>	17.7	1.7
Completeness [%]	100.0	98.5
Redundancy	54	30.3
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.59	291	200 nL Protein (30 mg/ml in 20 mM Tris HCl pH 9.0, 150 mM NaCl 1mM TCEP) 200 nL Mother Liquor (1.16M Li2SO4, 0.1M MES pH 6.59)