7NL4
OsNIP2;1 silicon transporter from rice
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.107 |
| Spacegroup name | P 1 |
| Unit cell lengths | 94.888, 96.692, 99.787 |
| Unit cell angles | 63.91, 71.16, 65.07 |
Refinement procedure
| Resolution | 48.780 - 3.000 |
| R-factor | 0.24 |
| Rwork | 0.238 |
| R-free | 0.28220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2evu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.019 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.010 | 2.910 |
| High resolution limit [Å] | 2.616 | 2.616 |
| Rmerge | 0.060 | 0.619 |
| Number of reflections | 48853 | 4644 |
| <I/σ(I)> | 6.2 | 1.5 |
| Completeness [%] | 91.8 | 79.6 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.980 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 28-32% PEG400 1 mM CdCl2 30 mM MgCl2.6H2O 0.1 M MES pH 6.5 |
