7NJF

Hen egg white lysozyme (HEWL) grown inside HARE serial crystallography chip

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	293
Detector technology	PIXEL
Collection date	2020-06-13
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9762
Spacegroup name	P 43 21 2
Unit cell lengths	78.500, 78.500, 38.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.510 - 1.700
R-factor	0.1754
Rwork	0.174
R-free	0.20280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dpx
RMSD bond length	0.006
RMSD bond angle	0.930
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.560	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	13833	1356
<I/σ(I)>	10.1
Completeness [%]	100.0
Redundancy	408
CC(1/2)	0.985	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	33% PEG 4000, 0.5 M NaCl, 50 mM sodium acetate pH 4.5