7NIO
Crystal structure of the SARS-CoV-2 helicase APO form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9126 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.699, 70.118, 83.998 |
| Unit cell angles | 104.31, 93.30, 112.16 |
Refinement procedure
| Resolution | 80.240 - 2.200 |
| R-factor | 0.2323 |
| Rwork | 0.230 |
| R-free | 0.28650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zsl |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.566 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.240 | 1.940 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmeas | 0.132 | |
| Number of reflections | 202027 | 8321 |
| <I/σ(I)> | 4.4 | 0.2 |
| Completeness [%] | 90.3 | |
| Redundancy | 2.5 | |
| CC(1/2) | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | containing 20 % Ethylene Glycol, 10 % PEG 8K, 0.05 M MES pH 6.5, 0.05 M Imidazole pH 6.5, 10% v/v Ethylene glycols mix |
