7NE9
A single sensor controls large variations in zinc quotas in a marine cyanobacterium
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.2827 |
| Spacegroup name | P 65 |
| Unit cell lengths | 129.250, 129.250, 77.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.625 - 2.100 |
| Rwork | 0.194 |
| R-free | 0.21780 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.608 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.300 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.193 | |
| Number of reflections | 43002 | 30185 |
| <I/σ(I)> | 19.4 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 39.8 | |
| CC(1/2) | 0.999 | 0.754 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 277 | 100 mM magnesium acetate, 100 mM MES pH 6, 16% (w/v) PEG 10000 |
