7NDK
Crystal structure of ZC3H12C PIN catalytic mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.753, 61.475, 111.620 |
| Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
| Resolution | 38.750 - 2.340 |
| R-factor | 0.2314 |
| Rwork | 0.229 |
| R-free | 0.27480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ndi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.269 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.750 | 2.390 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Number of reflections | 21746 | 1417 |
| <I/σ(I)> | 10.19 | |
| Completeness [%] | 97.0 | |
| Redundancy | 2.25 | |
| CC(1/2) | 0.998 | 0.629 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M KSCN, 20% PEG 3350 and 0.1 M Bis-Tris propane pH 6.5 |
