7NDE

Trichoderma parareesei PL7A beta-glucuronan lyase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 41 21 2
Unit cell lengths	69.589, 69.589, 111.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.550 - 1.450
R-factor	0.1556
Rwork	0.155
R-free	0.17380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cws
RMSD bond length	0.008
RMSD bond angle	0.985
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.550	1.500
High resolution limit [Å]	1.450	1.450
Number of reflections	49303	4791
<I/σ(I)>	22.47
Completeness [%]	100.0
Redundancy	26.2
CC(1/2)	1.000	0.440

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	2.4 M disodium malonate pH 7