7NCV
Crystal structure of reduced glutaredoxin 2 from Chlamydomonas reinhardtii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 45.648, 45.648, 104.667 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.530 - 1.500 |
| R-factor | 0.1541 |
| Rwork | 0.153 |
| R-free | 0.17990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fls |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.369 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2-3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.530 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.043 | 0.356 |
| Rmeas | 0.049 | 0.414 |
| Rpim | 0.023 | 0.202 |
| Number of reflections | 20838 | 2951 |
| <I/σ(I)> | 12.7 | 2.2 |
| Completeness [%] | 96.5 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 277 | 30% (w/v) PEG 8000 0.1 M sodium cacodylate pH 6.5 0.2 M sodium acetate |
