7NCT
Glutathione-S-transferase GliG mutant K127G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.430, 81.230, 126.980 |
| Unit cell angles | 90.00, 90.88, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.650 |
| R-factor | 0.2176 |
| Rwork | 0.214 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7nc3 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.163 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.750 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.060 | 0.637 |
| Number of reflections | 39163 | 4137 |
| <I/σ(I)> | 10.6 | 1.65 |
| Completeness [%] | 94.5 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Potassium thiocyanate, 30 % PEG 2000 MME |
