7NC9
Glutathione-S-transferase GliG mutant H26N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 267.820, 86.180, 63.640 |
| Unit cell angles | 90.00, 92.78, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.550 |
| R-factor | 0.2299 |
| Rwork | 0.228 |
| R-free | 0.26280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7nc3 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.183 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.650 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.066 | 0.576 |
| Number of reflections | 45771 | 4934 |
| <I/σ(I)> | 9.6 | 1.9 |
| Completeness [%] | 96.7 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M ammonium sulfate, 0.1 M bis-tris pH 6.5, 26 %PEG3350 |
