7NBZ
Crystal structure of ligand free open conformation of sulfoquinovosyl binding protein (SQBP) from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.321, 78.212, 83.456 |
| Unit cell angles | 109.04, 106.86, 104.77 |
Refinement procedure
| Resolution | 72.014 - 1.350 |
| Rwork | 0.183 |
| R-free | 0.20950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | D_1292108223 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.833 |
| Data reduction software | DIALS |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.014 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 234884 | 8434 |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 93.3 | |
| Redundancy | 2.1 | |
| CC(1/2) | 0.995 | 0.906 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 279 | 0.3 M Ammonium Acetate 0.1 M Bis-TRIS pH 5.5 25% w/v PEG 3350 |
