7NAZ

TPR-rich domain of EccA3 from M. smegmatis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976241
Spacegroup name	H 3
Unit cell lengths	159.001, 159.001, 43.364
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.940 - 1.600
R-factor	0.13575
Rwork	0.134
R-free	0.16994
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.417
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXD
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.940	1.440
High resolution limit [Å]	1.420	1.420
Rmerge	0.068
Rmeas	0.071
Rpim	0.027
Number of reflections	53900	75904
<I/σ(I)>	18.7
Completeness [%]	100.0	98.3
Redundancy	10
CC(1/2)	0.999	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	292.15	0.1 M MES pH6.5, 10 %(v/v) dioxane, 1.6 M ammonium sulfate