7N9V

CA-targeting nanobody is a tool for studying HIV-1 capsid lattice interactions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 1 21 1
Unit cell lengths	102.548, 140.378, 125.186
Unit cell angles	90.00, 113.90, 90.00

Refinement procedure

Resolution	46.880 - 3.450
R-factor	0.2147
Rwork	0.212
R-free	0.25750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 5O2U & 3H47
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.560
High resolution limit [Å]	3.450	9.480	3.500
Rmerge	0.110	0.050	0.822
Rmeas	0.134	0.062	1.005
Rpim	0.077	0.035	0.571
Number of reflections	39258	2004	1994
<I/σ(I)>	6.6
Completeness [%]	96.2	95.4	97.3
Redundancy	2.8	2.8	2.8
CC(1/2)		0.994	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		297	potassium nitrate, pH 6.9, 20% PEG3350