7N9I
1.4A Structure of Drosophila melanogaster Frataxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-15 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.904, 38.506, 46.297 |
| Unit cell angles | 90.00, 123.45, 90.00 |
Refinement procedure
| Resolution | 37.110 - 1.400 |
| R-factor | 0.1627 |
| Rwork | 0.162 |
| R-free | 0.18040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ekg |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.767 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.110 | 1.441 |
| High resolution limit [Å] | 1.400 | 1.405 |
| Rmerge | 0.037 | 0.328 |
| Number of reflections | 21840 | 1293 |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 95.9 | 83.72 |
| Redundancy | 3.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1:1 ratio of 7.2 mg/mL protein with 0.1M Citric Acid, pH5.0 and 1.6M Ammonium Sulfate |
