7N8V
Crystal structure of free ERI2 nuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.11583 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.134, 55.875, 75.751 |
| Unit cell angles | 90.00, 105.03, 90.00 |
Refinement procedure
| Resolution | 44.410 - 2.100 |
| R-factor | 0.2044 |
| Rwork | 0.203 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l83 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.511 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.112 | 0.583 |
| Number of reflections | 24036 | 1953 |
| <I/σ(I)> | 32.4 | |
| Completeness [%] | 82.1 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 15% MPEG 2K, 5% saturated MgSO4, 200 mM 5.6 Imidazole / Malate buffer, 5% Ethylene glycol |
