7N8T

Crystal Structure of AMP-bound Human JNK2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	65.388, 68.247, 98.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.090 - 1.690
R-factor	0.1916
Rwork	0.190
R-free	0.22030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3npc
RMSD bond length	0.008
RMSD bond angle	1.035
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.095	10.000
High resolution limit [Å]	1.690	1.693
Number of reflections	49432	49432
<I/σ(I)>	10.2
Completeness [%]	99.5
Redundancy	2.2
CC(1/2)	0.520	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2 M Potassium phosphate dibasic, 20% w/v Polyethylene glycol 3,350