7N8R

FGTGFG segment from the Nucleoporin p54, residues 63-68

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	15.710, 9.400, 23.260
Unit cell angles	90.00, 108.52, 90.00

Refinement procedure

Resolution	22.060 - 1.200
R-factor	0.146
Rwork	0.143
R-free	0.17450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.968
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	22.060	22.060	1.230
High resolution limit [Å]	1.200	5.360	1.200
Rmerge	0.186	0.184	0.386
Rmeas	0.215	0.213	0.454
Total number of observations	8482
Number of reflections	2137	29	143
<I/σ(I)>	5.43	7.72	2.63
Completeness [%]	98.8	96.7	92.3
Redundancy	3.969	3.31	3.133
CC(1/2)	0.972	0.984	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	ammonium sulfate