7N8J

Human importin alpha 1 in complex with Bimax2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.801, 84.706, 126.041
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.830 - 3.200
R-factor	0.2276
Rwork	0.227
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ukx
RMSD bond length	0.003
RMSD bond angle	0.549
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.830	3.420
High resolution limit [Å]	3.200	3.200
Rmerge	0.287	1.312
Rpim	1.010	0.464
Number of reflections	14410	2590
<I/σ(I)>	6.3	1.8
Completeness [%]	99.8	100
Redundancy	8.9	8.8
CC(1/2)	0.990	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.4 M Ammonium phosphate monobasic