7N7G
Crystal Structure of FosB from Enterococcus faecium with Fosfomycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-06-24 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 79.874, 79.874, 95.639 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.800 - 2.000 |
| R-factor | 0.2156 |
| Rwork | 0.213 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jh2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.641 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.800 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.102 | 0.745 |
| Number of reflections | 21556 | 2159 |
| <I/σ(I)> | 1.74 | |
| Completeness [%] | 99.7 | |
| Redundancy | 18.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20 mg/mL protein in 20mM HEPES pH7.5, 38% PEG MME 550, 150mM Magnesium Chloride |
