7N7B
crystal structure of the N-formyltrasferase HCAN_0200 from Helicobacter canadensis on complex with folinic acid and dTDP-3-aminofucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-18 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.264, 74.023, 82.996 |
| Unit cell angles | 90.00, 114.01, 90.00 |
Refinement procedure
| Resolution | 29.640 - 2.000 |
| R-factor | 0.1954 |
| Rwork | 0.192 |
| R-free | 0.25310 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 7n7a |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.520 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 45715 | 5967 |
| <I/σ(I)> | 13.5 | 2.8 |
| Completeness [%] | 96.6 | 93 |
| Redundancy | 5.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 11-14% PEG-8000, 2% DMSO, 100 mM homopipes (pH 5). Crystals soaked in 26% PEG-8000, 600 mM NaCl, 5 mM folinic acis, 10 mM dTDP-Fuc3N, 100 mM MES (pH 6) |
