7N7A
crystal structure of the dTDP-Qui3N N-formyltransferase from Helicobacter canadensis, apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-04 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.956, 74.701, 82.284 |
| Unit cell angles | 90.00, 113.74, 90.00 |
Refinement procedure
| Resolution | 29.670 - 1.800 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.20370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lxq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.618 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 65901 | 7018 |
| <I/σ(I)> | 18.7 | 3.9 |
| Completeness [%] | 99.3 | 99.7 |
| Redundancy | 7.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 11-14% PEG-8000, 2% DMSO, 100 mM homopipes (pH 5) |
