7N6O
The crystal structure of the GH30 subfamily 10 enzyme, AcXbh30A from Acetivibrio clariflavus in complex with xylobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.130, 107.494, 66.861 |
| Unit cell angles | 90.00, 89.88, 90.00 |
Refinement procedure
| Resolution | 34.200 - 1.500 |
| R-factor | 0.1786 |
| Rwork | 0.165 |
| R-free | 0.21360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7n6h |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.565 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.200 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.099 | 0.946 |
| Rmeas | 0.121 | 1.157 |
| Rpim | 0.069 | 0.657 |
| Number of reflections | 125373 | 6128 |
| <I/σ(I)> | 17.7 | 1.6 |
| Completeness [%] | 93.8 | 91.6 |
| Redundancy | 2.7 | 2.6 |
| CC(1/2) | 0.986 | 0.442 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 289 | 0.1M Sodium acetate 25% (w/v) PEG4000 |
