7N63
X-ray structure of HCAN_0200, an aminotransferase from Helicobacter canadensis in complex with its external aldimine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-24 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 61.005, 150.766, 91.058 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.710 - 1.400 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.18970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5u1z |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.782 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 81651 | 14740 |
| <I/σ(I)> | 12.7 | 2.7 |
| Completeness [%] | 98.6 | 96.3 |
| Redundancy | 6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | Enzyme incubated with 1 mM PLP and 5 mM dTDP-3-amino-3,6-dideoxyglucose. Precipitant = 15-18 % PEG 3350, 100 mM MgCl2, 100 mM MES, pH 6. |
