7N5T

ZBTB7A Zinc Finger Domain Bound to -200 Site of Fetal Globin Promoter (Oligo 5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 2 2 21
Unit cell lengths	35.479, 36.366, 176.377
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.370 - 2.900
R-factor	0.2498
Rwork	0.247
R-free	0.30930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7n5u
RMSD bond length	0.003
RMSD bond angle	0.498
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.370	2.990
High resolution limit [Å]	2.890	2.890
Rmerge	0.222	4.270
Rpim	0.033	0.736
Number of reflections	5536	542
<I/σ(I)>	24.7	2.1
Completeness [%]	99.3	97
Redundancy	41.3	17.4
CC(1/2)		0.873

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.2 M sodium bromide, 0.1 M Tris, pH 7.5, 25% PEG3350