7N5L

PCNA from Thermococcus gammatolerans: crystal II, collection 20, 3.07 A, 77.0 MGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 63
Unit cell lengths	187.684, 187.684, 64.116
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.635 - 3.070
R-factor	0.1548
Rwork	0.151
R-free	0.22370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a6d
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.635	3.180
High resolution limit [Å]	3.070	3.070
Rmerge	0.254	1.172
Number of reflections	24414	2386
<I/σ(I)>	12.45	2.26
Completeness [%]	99.7	99.92
Redundancy	10.2	9.8
CC(1/2)	0.996	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.2	291	100 mM sodium citrate pH 5.2, 3.2 M ammonium sulfate, 7.5% MPD